Phospho-Smad2 (Ser250) Rabbit Polyclonal Antibody

• Catalog Number: orb317976
• Category: Antibodies
• Description: Phospho-Smad2 (Ser250) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser250 EL(p-S)PT
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: SMAD2
• UniProt ID: Q15796
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: Smad2(phospho S250); p-Smad2(phospho S250); hMAD 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-phospho-Smad2 (Ser250) antibody (orb317976), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Anti-phospho-Smad2 (orb317976) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-p-Smad2 (Ser250) (orb317976) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 58kD, Observed band size: 58kD.