Phospho-Smad2 (Ser250) Rabbit Polyclonal Antibody

• Catalog Number: orb186236
• Category: Antibodies
• Description: Phospho-Smad2 (Ser250) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser250 EL(p-S)PT
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: SMAD2
• UniProt ID: Q15796
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Smad2(phospho S250); p-Smad2(phospho S250); hMAD 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Smad2 (Ser250) (orb186236) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Sample: Skin (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Smad2 (Ser250) (orb186236) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Blank control (blue line): HepG2 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-Smad2 (Ser250) antibody (orb186236), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.