Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody

SKU: orb158427

Description

Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad1 around the phosphorylation site of Ser465 SV(p-S)-NH2
• Target: SMAD1
• Molecular Weight: 51 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Smad1(phospho S465); Smad1(phospho Ser465); p-Smad1(Ser465); Mothers against decapentaplegic homolog 1; BSP 1; BSP1; BSP-1; Dwarfin A; DwfA; hSMAD 1; hSMAD1; JV 41; JV4 1; JV4-1; JV41; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; MADH 1; MADH1; Madr 1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; Sma and Mad related protein 1; SMAD 1; SMAD-1; SMAD family member 1; SMAD mothers against DPP homolog 1 Drosophila; Spinal muscular atrophy distal with upper limb predominance; TGF beta signaling protein 1; Transforming growth factor beta signaling protein 1; SMAD1_HUMAN; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1; Smad1; Transforming growth factor-beta-signaling protein 1.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (mono methyl K4)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Hela cell (UV) Lysates, Lane 2: Human A431 cell (UV) Lysates, Lane 3: Human U251 cell (UV) Lysates, Lane 4: Human 293T cell (TPA) Lysates, Primary: Anti-Phospho-Smad1 (orb158427) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kDa, Observed band size: 63kDa.

Quick Database Links

Gene Symbol

SMAD1

UniProt

Q15797