Phospho-SirT1 (Ser27) Rabbit Polyclonal Antibody

SKU: orb6958

Description

Phospho-SirT1 (Ser27) Rabbit Polyclonal Antibody

Research Area

Host-Virus Interaction, Immunology, Obesity, Virology, p53 Signaling Pathway

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SirT1 around the phosphorylation site of Ser27 AS(p-S)PA
• Target: SIRT1
• Molecular Weight: 58/81 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SIR2L1; NAD-dependent protein deacetylase sirtuin-1; EC:2.3.1.286; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1(hSIR2); SirtT1 75 kDa fragment; SIR1_HUMAN;

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Phospho-SirT1 (Ser27) antibody (orb6958), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

SIRT1

UniProt

Q96EB6

References

Xueru Wang 1 *, Kanglei Zhang 1 *, Waseem Ali *, Jiahui Li *, Qing Huang *, Dongdi Liu *, Gang Liu β, Di Ran † #, Zongping Liu Luteolin alleviates cadmium-induced metabolism disorder through antioxidant and anti-inflammatory mechanisms in chicken kidney Poultry Science, (2024)

PubMed:

Applications

WB

Reactivity

Gallus