Cart summary

You have no items in your shopping cart.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Catalog Number: orb6930

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6930
CategoryAntibodies
DescriptionPhospho-SGK1 (Ser78) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human SGK1 around the phosphorylation site of Ser78 PP(p-S)PS
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:25, IF=1:100-500, Flow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW49 kDa
TargetSGK1
UniProt IDO00141
RRIDAB_10933863
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesSGK1(phospho S78); p-SGK1(phospho S78); Serine/thr
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-SGK1 antibody (orb6930), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-SGK1 (Ser78)) polyclonal Antibody, Unconjugated (orb6930) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-SGK1 (Ser78)) Polyclonal Antibody, Unconjugated (orb6930) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-SGK1 (Ser78)) Polyclonal Antibody, Unconjugated (orb6930) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-SGK1 (Ser78)) Polyclonal Antibody, Unconjugated (orb6930) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Sample: A431 lysate at 30 ug, Primary: Rabbit Anti-Phospho-SGK1 (orb6930) at 1:300, Secondary:IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-Phospho-SGK1 (Ser78) (orb6930) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 49 kDa.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Sample: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-SGK1 (Ser78) (orb6930) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 50 kD.

Phospho-SGK1 (Ser78) Rabbit Polyclonal Antibody

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (orb6930) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.