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Phospho-SGK1 (Ser422) Rabbit Polyclonal Antibody

Catalog Number: orb6931

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6931
CategoryAntibodies
DescriptionPhospho-SGK1 (Ser422) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityCanine, Equine, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human SGK1 around the phosphorylation site of Ser422 GF(p-S)YA
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW49 kDa
TargetSGK1
UniProt IDO00141
RRIDAB_10941253
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesSGK1(phospho Ser422); SGK1(phospho S422); Serine/t
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-SGK1 (Ser422) Rabbit Polyclonal Antibody

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-SGK1 antibody (orb6931), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-SGK1 (Ser422) Rabbit Polyclonal Antibody

Sample: Lane 1: Pancreas (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Pancreas (Rat) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Phospho-SGK1 (Ser422) (orb6931) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45-60 kD, Observed band size: 50 kD.

Phospho-SGK1 (Ser422) Rabbit Polyclonal Antibody

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-SGK1 (Ser422) (orb6931) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Phospho-SGK1 (Ser422) Rabbit Polyclonal Antibody

Sample: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-SGK1 (Ser422) (orb6931) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 49 kD.

Phospho-SGK1 (Ser422) Rabbit Polyclonal Antibody

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-SGK1 (Ser422) Polyclonal Antibody, Unconjugated (orb6931) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.