Phospho-RPS6KA1 (Thr573) Rabbit Polyclonal Antibody

SKU: orb128681

Description

Phospho-RPS6KA1 (Thr573) Rabbit Polyclonal Antibody

Research Area

MAPK/ERK Signalling Pathway

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RPS6KA1 around the phosphorylation site of Thr573 LM(p-T)P
• Target: RPS6KA1
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

KS6A1_HUMAN; Ribosomal protein S6 kinase alpha-1; EC:2.7.11.1; MAPKAPK1A; RSK1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1(p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a(MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1(RSK-1); ribosomal protein S6 kinase A1; RSK; HU-1; p90Rsk; MAPKAPK1; RSK1 p90;

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P-RPS6KA1 (Thr573)) Polyclonal Antibody, Unconjugated (orb128681) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Uterus tissue lysates, Lane 3: Mouse Spleen tissue lysates, Primary: Anti-Phospho-RPS6KA1 (Thr573) (orb128681) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kDa, Observed band size: 90 kDa.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-p90RSK (Thr573) Polyclonal Antibody, Unconjugated (orb128681) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

RPS6KA1