Phospho-Rb (Ser807 + Ser811) Rabbit Polyclonal Antibody

• Catalog Number: orb6861
• Category: Antibodies
• Description: Phospho-Rb (Ser807 + Ser811) Rabbit Polyclonal Antibody
• Target: RB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of Ser807/Ser811 YI(p-S)PLK(p-S)PY
• UniProt ID: P06400
• RRID: AB_10938714
• MW: 102 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Rb(phospho-Ser807); Rb(phospho S807); p-Rb(phospho
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse eye), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Rb (Ser807 + Ser811)) Polyclonal Antibody, Unconjugated (orb6861) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.