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Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody

Catalog Number: orb6843

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6843
CategoryAntibodies
DescriptionPhospho-RAF1 (Ser296) Rabbit Polyclonal Antibody
TargetRAF1
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityBovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human RAF1 around the phosphorylation site of Ser296 SS(p-S)PN
UniProt IDP04049
RRIDAB_10921646
MW73 kDa
Tested applicationsFC
Dilution rangeFlow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesRAF1(phospho S296); RAF1(phospho Ser296); murine l
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NoteFor research use only
Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-phospho-c-Raf (Ser296) antibody (orb6843), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

  • Phospho-Raf1 (Ser296) Antibody [orb1925497]

    WB

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (Cy3) [orb988746]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Cy3

    100 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (FITC) [orb9691]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    FITC

    100 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (PE-Cy5) [orb886571]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    PE/Cy5

    100 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (BF750) [orb1595464]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    BF750

    100 μl