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Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody

Catalog Number: orb6843

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb6843
CategoryAntibodies
DescriptionPhospho-RAF1 (Ser296) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC
Predicted ReactivityBovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human RAF1 around the phosphorylation site of Ser296 SS(p-S)PN
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeFlow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW73 kDa
TargetRAF1
UniProt IDP04049
RRIDAB_10921646
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesRAF1(phospho S296); RAF1(phospho Ser296); murine l
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-phospho-c-Raf (Ser296) antibody (orb6843), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

  • Phospho-Raf1 (Ser296) Antibody [orb1925497]

    WB

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (Cy5) [orb900273]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Cy5

    100 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (PE-Cy5.5) [orb902434]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    PE/Cy5.5

    100 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (APC) [orb1006814]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    APC

    100 μl
  • Phospho-RAF1 (Ser296) Rabbit Polyclonal Antibody (Cy5.5) [orb1047455]

    FC

    Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Cy5.5

    100 μl