Phospho-Raf1 (Ser259) Antibody

• Catalog Number: orb1733067
• Category: Antibodies
• Description: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1
• Concentration: 1mg/ml
• Dilution range: WB: 1/500-1/1000; IHC: 1/50-1/100
• Conjugation: Unconjugated
• MW: Calculated MW: 73 kDa; Observed MW: 73 kDa
• Entrez: 5894
• UniProt ID: P04049
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: RAF1; RAF; RAF proto-oncogene serine/threonine-pro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.