Phospho-RAC1 + RAC2 (Ser71) Rabbit Polyclonal Antibody

• Catalog Number: orb4705
• Category: Antibodies
• Description: Phospho-RAC1 + RAC2 (Ser71) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RAC1 around the phosphorylation site of Ser71 PL(p-S)YP
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: RAC1
• UniProt ID: P63000
• RRID: AB_10936825
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: RAC1(phospho S71); RAC2(phospho S71); RAC1+RAC2(ph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: Human FHC cells, 4% Paraformaldehyde-fixed, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-RAC1+RAC2 (Ser71) Polyclonal Antibody, Unconjugated (orb4705) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-RAC1+RAC2 (Ser71)) polyclonal Antibody, Unconjugated (orb4705) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.