Phospho-RAC1 + RAC2 (Ser71) Rabbit Polyclonal Antibody

SKU: orb4705

Description

Phospho-RAC1 + RAC2 (Ser71) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC
• Dilution range: ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RAC1 around the phosphorylation site of Ser71 PL(p-S)YP
• Target: RAC1
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

RAC1(phospho S71); RAC2(phospho S71); RAC1+RAC2(phospho Ser71); Cell migration inducing gene 5 protein; Cell migration-inducing gene 5 protein; MIG5; MIG 5; Migration inducing gene 5; Migration inducing protein 5; p21 Rac1; Rac 1; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1; Rho family small GTP binding protein Rac1; TC 25; TC25; MGC111543; p21-Rac1; RAC1; RAC1_HUMAN; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; rho family small GTP binding protein Rac1.

Tissue/Cell: Human FHC cells, 4% Paraformaldehyde-fixed, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-RAC1+RAC2 (Ser71) Polyclonal Antibody, Unconjugated (orb4705) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-RAC1+RAC2 (Ser71)) polyclonal Antibody, Unconjugated (orb4705) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

RAC1

UniProt

P63000