Phospho-Progesterone Receptor (Ser190) Rabbit Polyclonal Antibody

SKU: orb704511

Description

Phospho-Progesterone Receptor (Ser190) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from rat Progesterone Receptor around the phosphorylation site of Ser190 GL(p-S)P
• Target: PGR
• Molecular Weight: 99 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PGR(phospho S190); NR3C3; Nuclear receptor subfamily 3 group C member 3; PGR; PR; PRA; PRB; Progesterone receptor; Progestin receptor form A; Progestin receptor form B; PRGR_RAT.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Phospho-Progesterone Receptor (Ser190) antibody (orb704511), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Progesterone Receptor (Ser190) (orb704511) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD.

Sample: Lane 1: Uterus (Mouse) Lysate at 40 ug, Lane 2: Testis (Mouse) Lysate at 40 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: MCF-7 (Human) Cell Lysate at 30 ug, Lane 5: HepG2 (Human) Cell Lysate at 30 ug, Lane 6: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-progesterone receptor (orb704511) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 118/82-88 kD, Observed band size: 120 kD.

Quick Database Links

Gene Symbol

PGR

UniProt

Q63449