Phospho-PRKD3 (Ser41) Rabbit Polyclonal Antibody

• Catalog Number: orb4440
• Category: Antibodies
• Description: Phospho-PRKD3 (Ser41) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Rabbit
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PRKD3 around the phosphorylation site of Ser41 RL(p-S)NG
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100 kDa
• Target: PRKD3
• UniProt ID: O94806
• RRID: AB_10953848
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PRKD3(phospho S41); PKD/PKCμ(phospho-Ser41); EPK 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

ST Lim, X Zhao, S Liu, W Zhang, Y Tan, N Mullappilly, SM Swain, ML Leong… LRG1 inhibition promotes acute pancreatitis recovery by inducing cholecystokinin Type 1 receptor expression via Akt Theranostics, (2025)

PubMed:

Applications

WB

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-PRKD3 (Ser41)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-PRKD3 (Ser41)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.