Phospho-PLK1 (Ser137) Rabbit Polyclonal Antibody

• Catalog Number: orb6763
• Category: Antibodies
• Description: Phospho-PLK1 (Ser137) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137 R(p-S)LL
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:25, IF=1:100-500, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: plk1
• UniProt ID: P53350
• RRID: AB_10927807
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PLK1(phospho S137); p-PLK1(phospho S137); PLK1(pho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Phospho-PLK1 (Ser137) antibody (orb6763), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-PLK1 (Ser137)) polyclonal Antibody, Unconjugated (orb6763) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat colon tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLK1 (Ser137)) Polyclonal Antibody, Unconjugated (orb6763) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human A549 cell lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human K562 cell lysates, Lane 5: Human U-2 OS cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human MDA-MB-231 cell lysates, Primary: Anti-Phospho-PLK1 (Ser137) (orb6763) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 60 kDa.

Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-PLK1 (Ser137) (orb6763) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.