Phospho-PLK1 (Ser137) Rabbit Polyclonal Antibody

SKU: orb6763

Description

Phospho-PLK1 (Ser137) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137 R(p-S)LL
• Target: plk1
• Molecular Weight: 68 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PLK1(phospho S137); p-PLK1(phospho S137); PLK1(phospho-Thr137); PLK1(phospho Thr137); p-PLK1(Thr137); p-PLK1(T137); Polo-Like Kinase(phospho T137); PLK 1; PLK; polio like kinase; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase 13; Serine/threonine protein kinase PLK1; Serine/threonine-protein kinase; STPK 13; STPK13; Polo like kinase kinase; Cell cycle regulated protein kinase; PLK-1; PLK1_HUMAN.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Phospho-PLK1 (Ser137) antibody (orb6763), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-PLK1 (Ser137)) polyclonal Antibody, Unconjugated (orb6763) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat colon tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLK1 (Ser137)) Polyclonal Antibody, Unconjugated (orb6763) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human A549 cell lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human K562 cell lysates, Lane 5: Human U-2 OS cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human MDA-MB-231 cell lysates, Primary: Anti-Phospho-PLK1 (Ser137) (orb6763) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 60 kDa.

Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-PLK1 (Ser137) (orb6763) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.

Quick Database Links

Gene Symbol

plk1

UniProt

P53350