Phospho-PLCG1 (Tyr783) Rabbit Polyclonal Antibody

SKU: orb6046

Description

Phospho-PLCG1 (Tyr783) Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Canine, Equine, Gallus, Human, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr783 GF(p-Y)VE
• Target: PLCG1
• Molecular Weight: 148 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLCG1 (p-Y783); p-PLCG1; phospho-PLCG1; IDAA; NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1; Cded; Plc-1; Plc-gamma1; Plcg-1; PPLCA; PLCG1_HUMAN; PLCG1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II (PLC-II); Phospholipase C-gamma-1 (PLC-gamma-1); 3.1.4.11; PLCG1_MOUSE; PLCG1_RAT;

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PLC gamma 1 (Tyr783)) Polyclonal Antibody, Unconjugated (orb6046) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PLCγ1 Polyclonal Antibody, Unconjugated (orb6046) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat rectal cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PLCγ1 Polyclonal Antibody, Unconjugated (orb6046) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PLCG1

UniProt

P19174