Phospho-PKC gamma (Thr514) Rabbit Polyclonal Antibody

SKU: orb6746

Description

Phospho-PKC gamma (Thr514) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Equine, Gallus, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PKC gamma around the phosphorylation site of Thr514 TR(p-T)FC
• Target: PRKCG
• Molecular Weight: 78 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKCG | PKC gamma (p-T514); p-PKC gamma; phospho-PKC gamma; PKC-gamma; PKCC; PKCG; PKCI(3); PKCgamma; SCA14; Prkcc; PKC; PKCI; Prkc; RATPKCI; KPCG_HUMAN; PRKCG; 2.7.11.13; KPCG_MOUSE; KPCG_RAT;

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PKC gamma (Thr514)) Polyclonal Antibody, Unconjugated (orb6746) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PRKCG

UniProt

P05129