Phospho-PKC alpha (Thr638) Rabbit Polyclonal Antibody

SKU: orb6739

Description

Phospho-PKC alpha (Thr638) Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Serine/Threonine Kinase Pathway, Serine/Threonine Kinases, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human PKC alpha around the phosphorylation site of Thr638 VL(p-T)PP
• Target: PRKCA
• Molecular Weight: 77 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PKC alpha(phospho T638); phospho-PKC beta II(Thr641); PKC(phospho-Thr641); phospho-PKC alpha/beta II(Thr638/641); phospho-PKC alpha/beta II(Thr638+641); PKC alpha; AAG6; Aging associated gene 6; PICK 1; PKC A; PKC alpha; PKCA; PRKACA; PRKC A; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type; KPCB_HUMAN; PKC α.

Paraformaldehyde-fixed, paraffin embedded (Rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PKC alpha beta II (Thr638 641)) Polyclonal Antibody, Unconjugated (orb6739) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PKC alpha beta II (Thr638 641)) Polyclonal Antibody, Unconjugated (orb6739) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Phospho-PKC alpha/beta II (Thr638/641) (orb6739) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 77 kD, Observed band size: 77 kD.

Quick Database Links

Gene Symbol

PRKCA

UniProt

P17252