Phospho-PFKM/PFK1 (Ser775) Rabbit Polyclonal Antibody

• Catalog Number: orb317742
• Category: Antibodies
• Description: Phospho-PFKM/PFK1 (Ser775) Rabbit Polyclonal Antibody
• Target: PFKM
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PFKM around the phosphorylation site of Ser775 KR(p-S)GE
• UniProt ID: P08237
• MW: 86 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PFKM(phospho S775); PFKM(phospho Ser775); p-PFKM(S
• Note: For research use only

Sample: Lane 1: Human A431 cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human Raji cell lysates, Lane 4: Human SH-SY5Y cell lysates, Lane 5: Human LOVO cell lysates, Lane 6: Human MCF-7 cell lysates, Primary: Anti-phospho-PFKM/PFK1 (Ser775) (orb317742) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 86 kDa, Observed band size: 77 kDa.

Tissue/Cell: Rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PFKL (Ser775) Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.