Phospho-PDHA1 (Ser293) Rabbit Polyclonal Antibody

SKU: orb6670

Description

Phospho-PDHA1 (Ser293) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Energy Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

• Tested Applications: WB
• Dilution range: WB=1:500-2000
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293 GH(p-S)MS
• Target: PDHA1
• Molecular Weight: 40 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PDHA1(phospho Ser293); PDHA1(phospho S293); mitochondrial; somatic form; ODPA_HUMAN; PDH; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase(lipoamide) alpha 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Muscle tissue lysates, Lane 3: Mouse Heart tissue lysates, Lane 4: Mouse Liver tissue lysates, Lane 5: Rat Muscle tissue lysates, Primary: Anti-phospho-PDHA1 (Ser293) (orb6670) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 48 kDa.

Quick Database Links

Gene Symbol

PDHA1

UniProt

P08559

References

Eleftheriadis, Theodoros et al. Inhibition of indoleamine 2,3-dioxygenase in mixed lymphocyte reaction affects glucose influx and enzymes involved in aerobic glycolysis and glutaminolysis in alloreactive T-cells Hum Immunol, 74, 1501-1509 (2013)

PubMed: 23993986