Phospho-Paxillin (Ser83) Rabbit Polyclonal Antibody

• Catalog Number: orb317745
• Category: Antibodies
• Description: Phospho-Paxillin (Ser83) Rabbit Polyclonal Antibody
• Target: PXN
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from rat Paxillin around the phosphorylation site of Ser83 PP(p-S)PS
• UniProt ID: Q66H76
• MW: 68 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PXN(phospho S83); Paxillin(phospho Ser83); Paxilli
• Research Area: Cell Biology, Cytoplasmic, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Paxillin (Ser83)) Polyclonal Antibody, Unconjugated (orb317745) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Paxillin (Ser83)) Polyclonal Antibody, Unconjugated (orb317745) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.