Phospho-PAK1/2/3 (Thr423) Rabbit Polyclonal Antibody

SKU: orb11231

Description

Phospho-PAK1/2/3 (Thr423) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Thr423 RS(p-T)MV
• Target: PAK1
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PAK1+PAK2+PAK3(phospho T423); PAK1/2/3(phospho Thr423); PAK1/2/3(phospho T423); alpha PAK; beta PAK; gamma PAK; Oligophrenin 3; OPHN3; p21 activated kinase 1; p21 Activated Kinase 1+2+3; p21 activated kinase 2; p21 activated kinase 3; P65 PAK; PAK1; PAK2; PAK3; PAK3beta; PAK65; PAKalpha; PAKgamma; S6/H4 kinase; Serine/threonine protein kinase PAK 1; Serine/threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 3; Serine/threonine protein kinase PAK1; Serine/threonine protein kinase PAK2; Serine/threonine protein kinase PAK3; PAK1_HUMAN; PAK2_HUMAN; PAK3_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PAK1/2/3 (Thr423)) Polyclonal Antibody, Unconjugated (orb11231) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PAK1

UniProt

Q13153