Phospho-p70 S6 Kinase Beta (Thr228) Rabbit Polyclonal Antibody

• Catalog Number: orb6618
• Category: Antibodies
• Description: Phospho-p70 S6 Kinase Beta (Thr228) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human RPS6KB2 around the phosphorylation site of Thr228 TH(p-T)FC
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: RPS6KB2
• UniProt ID: Q9UBS0
• RRID: AB_10925863
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: P70 S6 Kinase beta(phospho Thr228); P70 S6 Kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Eye (Mouse) Lysate at 40 ug, Primary: Anti-phospho-p70 S6 Kinase Beta (Thr228) (orb6618) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 59 kD, Observed band size: 59 kD.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Phospho-p70 S6 Kinase Beta (Thr228) antibody (orb6618), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.