Phospho-p107 (Thr369) Rabbit Polyclonal Antibody

• Catalog Number: orb100309
• Category: Antibodies
• Description: Phospho-p107 (Thr369) Rabbit Polyclonal Antibody
• Target: RBL1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Thr369 PS(p-T)PL
• UniProt ID: P28749
• MW: 121 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: p107(phospho T369); p-p107(phospho T369); RBL1(pho
• Research Area: Cancer Research, Nuclear Signaling Pathway, RB Fam
• Note: For research use only

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-p107 (Thr369) antibody (orb100309), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-APC, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-p107 (Thr369) Polyclonal Antibody, Unconjugated (orb100309) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human 293T cell lysates, Lane 6: Human U-2 OS cell lysates, Primary: Anti-phospho-p107 (Thr369) (orb100309) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 130 kDa.