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Phospho-Nucleolin (Thr84) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb2561195
| Catalog Number | orb2561195 |
|---|---|
| Category | Antibodies |
| Description | Phospho-Nucleolin (Thr84) Recombinant Rabbit Monoclonal Antibody |
| Target | NCL |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Human |
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | A synthesized peptide derived from human NCL around the phosphorylation site of T84 AV-pT-PG |
| UniProt ID | P19338 |
| MW | 100 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-1:2000, IHC-P=1:100-1:200, IHC-F=1:100-1:200, IF=1:100-1:200 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | NCL; Nucl; Nucleolin; Protein C23. |
| Research Area | DNA and RNA Research, Epigenetics and Nuclear Sign Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of (1) K562 cell lysate, (2) K562 cell treated with Calyculin A lysate. Using Phospho-Nucleolin (Thr84) (orb2561195) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
