Phospho-nNOS (Ser847) Rabbit Polyclonal Antibody

SKU: orb221792

Description

Phospho-nNOS (Ser847) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Xenopus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse nNOS around the phosphorylation site of Ser847 FN(p-S)VS
• Target: NOS1
• Molecular Weight: 160 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

nNOS(neuronal)(phospho S847); p-nNOS(neuronal)(phospho S847); nNOS(phospho S847); p-nNOS(phospho S847); nNOS(neuronal)(phospho S852)(human); NOS1_HUMAN; Nitric oxide synthase, brain; NOS1; EC:1.14.13.39; Constitutive NOS; N NOS; N-NOS; NC-NOS; NOS type I; Neuronal NOS(N-NOS; nNOS); Peptidyl-cysteine S-nitrosylase NOS1; bNOS; neuronal Nitric Oxide Synthase;

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-nNOS Polyclonal Antibody, Unconjugated (orb221792) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

NOS1