Phospho-NMDAR2A (Tyr1325) Rabbit Polyclonal Antibody

SKU: orb6537

Description

Phospho-NMDAR2A (Tyr1325) Rabbit Polyclonal Antibody

Research Area

NMDA receptors, Neuroscience

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Gallus, Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NMDAR2A around the phosphorylation site of Tyr1325 NF(p-Y)GS
• Target: GRIN2A
• Molecular Weight: 165 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

NMDAR2A(phospho Y1325); p-NMDAR2A(phospho Y1325); Glutamate [NMDA] receptor subunit epsilon 1; Glutamate [NMDA] receptor subunit epsilon-1; Glutamate receptor; Glutamate receptor ionotropic N methyl D aspartate 2A; GRIN 2A; GRIN2A; HNR2A; N methyl D aspartate receptor channel, subunit epsilon 1; N Methyl D Aspartate Receptor Subtype 2A; N methyl D aspartate receptor subunit 2A; N-methyl D-aspartate receptor subtype 2A; NMDA receptor subtype 2A; NMDA Receptor Type 2A; NMDAR 2A; NMDAR2A; NMDE1_HUMAN; NR 2A; NR2A; OTTHUMP00000160135; OTTHUMP00000174531.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-NMDAR2A (Tyr1325)) Polyclonal Antibody, Unconjugated (orb6537) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-NMDAR2A (Tyr1325)) Polyclonal Antibody, Unconjugated (orb6537) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

GRIN2A

UniProt

Q12879