Phospho-NLK (Thr298) Rabbit Polyclonal Antibody

• Catalog Number: orb157946
• Category: Antibodies
• Description: Phospho-NLK (Thr298) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298 HM(p-T)QE
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: NLK
• UniProt ID: Q9UBE8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Nemo-like kinase(phospho-Thr298); Nemo-like kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

F Nasehi 1, C Rylance 1, E Schnell 2, M Greene 3, C Conway 4, Z Hough 5, S Duckett 3, R Muise-Helmericks 6, A C Foley Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle Biol Open, (2024)

PubMed: 39211992

Applications

WB

Reactivity

Mouse

Wilkes, M. C. et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase Nat Commun, 11, 3344 (2020)

PubMed: 32620751

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Nemo-like kinase (Thr298)) Polyclonal Antibody, Unconjugated (orb157946) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Nemo-like kinase (Thr298) (orb157946) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.