Phospho-NLK (Thr298) Rabbit Polyclonal Antibody

SKU: orb157946

Description

Phospho-NLK (Thr298) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298 HM(p-T)QE
• Target: NLK
• Molecular Weight: 58 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RGD1561602; NLK_HUMAN; NLK; Nemo-like kinase; Protein LAK1; 2.7.11.24; LAK1; NLK_MOUSE; NLK_RAT; nemo like kinase; NLK (phospho-T298); p-NLK; phospho-NLK; NLK (phospho-Thr298)

Quick Database Links

Gene Symbol

NLK

UniProt

Q9UBE8

F Nasehi 1, C Rylance 1, E Schnell 2, M Greene 3, C Conway 4, Z Hough 5, S Duckett 3, R Muise-Helmericks 6, A C Foley Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle Biol Open, (2024)

PubMed: 39211992

Applications

WB

Reactivity

Mouse

Wilkes, M. C. et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase Nat Commun, 11, 3344 (2020)

PubMed: 32620751