Phospho-NLK (Thr298) Rabbit Polyclonal Antibody

SKU: orb157946

Description

Phospho-NLK (Thr298) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Nuclear Signaling Pathway, Phosphatases

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298 HM(p-T)QE
• Target: NLK
• Molecular Weight: 58 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Nemo-like kinase(phospho-Thr298); Nemo-like kinase(phospho-T298); p-Nemo-like kinase(Thr298); p-Nemo-like kinase(T298); DKFZp761G1211; FLJ21033; LAK1; Nemo like kinase; Nemo-like kinase; Nlk; NLK_HUMAN; Protein LAK1; Serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Nemo-like kinase (Thr298)) Polyclonal Antibody, Unconjugated (orb157946) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Nemo-like kinase (Thr298) (orb157946) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.

Quick Database Links

Gene Symbol

NLK

UniProt

Q9UBE8

References

F Nasehi 1, C Rylance 1, E Schnell 2, M Greene 3, C Conway 4, Z Hough 5, S Duckett 3, R Muise-Helmericks 6, A C Foley Analysis of potential TAK1/Map3k7 phosphorylation targets in hypertrophy and cachexia models of skeletal muscle Biol Open, (2024)

PubMed: 39211992

Applications

WB

Reactivity

Mouse

Wilkes, M. C. et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase Nat Commun, 11, 3344 (2020)

PubMed: 32620751