Phospho-NFKB p65 (Thr505) Rabbit Polyclonal Antibody

• Catalog Number: orb6508
• Category: Antibodies
• Description: Phospho-NFKB p65 (Thr505) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human around the phosphorylation site of Thr505 LV(p-T)G
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: RELA
• UniProt ID: Q04206
• RRID: AB_10940942
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NFκ B-p65(phospho-Thr505); NFκ B-p65(phospho-Thr50
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr505) antibody (orb6508), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Hcclm3 Cell Lysate at 30 ug, Primary: Anti-phospho-NFKB p65 (Thr505) (orb6508) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kD, Observed band size: 61/58 kD.

Sample: Molt-4 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-NFKB p65 (Thr505) (orb6508) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 62 kD.

Sample: Pancreas (Mouse) Lysate at 40 ug, HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-NFKB p65 (Thr505) (orb6508) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.