Phospho-NFATc2 (Ser330) Rabbit Polyclonal Antibody

SKU: orb101236

Description

Phospho-NFATc2 (Ser330) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NFATc2 around the phosphorylation site of Ser330 DP(p-S)PV
• Target: NFATC2
• Molecular Weight: 100 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

NFATC2(phospho Ser330); NFATC2(phospho S330); AI607462; KIAA0611; NF ATp; NF-ATc2; NFAT 1; NFAT pre existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1-D; NFATc2; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2; Nuclear factor of activated T cells pre-existing component; T cell transcription factor NFAT; NF2IP_HUMAN; Nuclear factor of activated T-cells, cytoplasmic 2; NFAT pre-existing subunit; NF-ATp; T-cell transcription factor NFAT1.

Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-phospho-NFATc2 (Ser330) antibody (orb101236), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (phospho-NFATc2 (Ser330)) Polyclonal Antibody, Unconjugated (orb101236) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Human Raji cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human U251 cell lysates, Primary: Anti-phospho-NFATc2 (Ser330) (orb101236) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100 kDa, Observed band size: 140 kDa.

Quick Database Links

Gene Symbol

NFATC2

UniProt

Q13469

References

Minghui Wang a b 1 , Huiying Cai a b 1 , Manman Wang c 1 , Honggang Gao b 1 , Xiaona Zhao b d , Ruixue Rong a b , Jing Zhang b , Zejin Zhang b e , Guoqing Liu a b , Tao Yuan b f , Fangjie Xia b d , Lin Zhu a b , Zhenzhen Yan a b , Xinxin Kong b d , Wei Qin Lonicerin targets ADRA1D and RSPO3 to ameliorate diabetes-induced vascular injury through Ca2+/Calcineurin/NFAT1-dependent anti-EndMT pathway Phytomedicine, (2025)

PubMed: