Phospho-NFATc2 (Ser326) Rabbit Polyclonal Antibody

• Catalog Number: orb6499
• Category: Antibodies
• Description: Phospho-NFATc2 (Ser326) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NFATc2 around the phosphorylation site of Ser326 KT(p-S)PD
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1-2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 102 kDa
• Target: NFATC2
• UniProt ID: Q13469
• RRID: AB_10941890
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AI607462; KIAA0611; NF ATp; NF-ATc2; NFAT 1; NFAT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Molt4. Primary Antibody (green line): Rabbit Anti-phospho-NFATc2 (Ser326) antibody (orb6499), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): U251. Primary Antibody (green line): Rabbit Anti-phospho-NFATc2 (Ser326) antibody (orb6499), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-NFATc2 (Ser326) Antibody (orb6499) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).