Phospho-NF2 (Ser518) Rabbit Polyclonal Antibody

• Catalog Number: orb6495
• Category: Antibodies
• Description: Phospho-NF2 (Ser518) Rabbit Polyclonal Antibody
• Target: NF2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Merlin around the phosphorylation site of Ser518 RL(p-S)ME
• UniProt ID: P35240
• RRID: AB_10938584
• MW: 70 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Merlin(phospho-Ser518); NF2(phospho S518); NF2/Mer
• Research Area: Cancer Research, Cell Biology, Cytoplasmic, Neuros
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Phospho-NF2 (Ser518) (orb6495) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-NF2 (Ser518) Polyclonal Antibody, Unconjugated (orb6495) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.