Phospho-NDRG1 (Ser330) Rabbit Polyclonal Antibody

• Catalog Number: orb6482
• Category: Antibodies
• Description: Phospho-NDRG1 (Ser330) Rabbit Polyclonal Antibody
• Target: NDRG1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NDRG1 around the phosphorylation site of Ser330 TA(p-S)GS
• UniProt ID: Q92597
• RRID: AB_10925251
• MW: 43 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NDRG1(phospho S330); P-NDRG1(Ser330); N-myc downst
• Note: For research use only

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-NDRG1 (Ser330) (orb6482) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

Tissue/Cell: Mouse embryos tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-NDRG1 (Ser330) Polyclonal Antibody, Unconjugated (orb6482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.