Phospho-MYPT1 (Thr696) Rabbit Polyclonal Antibody

• Catalog Number: orb6993
• Category: Antibodies
• Description: Phospho-MYPT1 (Thr696) Rabbit Polyclonal Antibody
• Target: PPP1R12A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MYPT1 around the phosphorylation site of Thr696 RS(p-T)QG
• UniProt ID: O14974
• RRID: AB_10926512
• MW: 113 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MYPT1(phospho Thr696); MYPT1(phospho T696); Myosin
• Note: For research use only

Blank control (blue): Hela cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-MYPT1 (Thr696) antibody (orb6993), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: 293T (human) cell Lysate at 40 ug, Primary: Anti-p-MYPT1 (Thr696) (orb6993) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113kD, Observed band size: 120 kD.

Sample: BRL-3A (Rat) cell Lysate at 40 ug, Primary: Anti-p-MYPT1 (Thr696) (orb6993) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113kD, Observed band size: 120 kD.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human MCF-7 cell lysates, Primary: Anti-Phospho-MYPT1 (Thr696) (orb6993) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113 kDa, Observed band size: 125 kDa.