Phospho-MYPT1 (Thr696) Rabbit Polyclonal Antibody

• Catalog Number: orb317659
• Category: Antibodies
• Description: Phospho-MYPT1 (Thr696) Rabbit Polyclonal Antibody
• Target: PPP1R12A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MYPT1 around the phosphorylation site of Thr696 RS(p-T)QG
• UniProt ID: O14974
• MW: 113 kDa
• Tested applications: FC, ICC
• Dilution range: ICC/IF=1:100, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Myosin Phosphatase(phospho T696); Myosin Phosphata
• Note: For research use only

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-MYPT1 (Thr696) antibody (orb317659), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-MYPT1 (Thr696)) polyclonal Antibody, Unconjugated (orb317659) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.