Phospho-Mst2 (Thr180) Rabbit Polyclonal Antibody

SKU: orb100863

Description

Phospho-Mst2 (Thr180) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Mst2 around the phosphorylation site of Thr180 RN(p-T)VI
• Target: STK3
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

STK3 | Mst2 (p-T180); p-Mst2; phospho-Mst2; KRS1; MST2; KRS2; MST1; YSK3; STK4_HUMAN; STK4; Mammalian STE20-like protein kinase 1 (MST-1); STE20-like kinase MST1; Serine/threonine-protein kinase Krs-2; 2.7.11.1; STK3_HUMAN; STK3; Mammalian STE20-like protein kinase 2 (MST-2); STE20-like kinase MST2; Serine/threonine-protein kinase Krs-1; STK3_RAT;

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Mst2 (Thr180) (orb100863) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Tissue/cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Phospho-Mst2 Polyclonal Antibody, Unconjugated (orb100863) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: Mouse Placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Phospho-Mst2 Polyclonal Antibody, Unconjugated (orb100863) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

STK3

UniProt

Q13043