Phospho-MSK1 (Ser360) Rabbit Polyclonal Antibody

SKU: orb6444

Description

Phospho-MSK1 (Ser360) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Kinases, Oxidative Stress, Receptor Tyrosne Kinases

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MSK1 around the phosphorylation site of Ser360 TY(p-S)PA
• Target: RPS6KA5
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MSK1(phospho S360); MSK1(phospho-Ser360); MSK1(phospho-S360); p-MSK1(Ser360); Pp-MSK1(S360); MSK1; RPS6KA5; 90 kDa ribosomal protein S6 kinase 5; EC 2.7.11.1; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen-and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MSK1 (Ser360)) Polyclonal Antibody, Unconjugated (orb6444) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lymph node lyates (mo), Primary: Rabbit Anti-Phospho-MSK1 (Ser360) (orb6444) at 1:300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 90 kD, Observed band size: 100 kD.

Tissue/Cell: Mouse bain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MSK1 Polyclonal Antibody, Unconjugated (orb6444) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

RPS6KA5

UniProt

O75582