Phospho-MEK7 (Ser271 + Thr275) Rabbit Polyclonal Antibody

• Catalog Number: orb6592
• Category: Antibodies
• Description: Phospho-MEK7 (Ser271 + Thr275) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MKK7 around the phosphorylation site of Ser271/Thr275 VD(p-S)KAK(p-T)RS
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: MAP2K7
• UniProt ID: O14733
• RRID: AB_10933514
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MEK7(phospho S271+T275); p-MEK7(phospho S271+T275)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Jurkat cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-MEK7 (Ser271 + Thr275) antibody (orb6592), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (P-MEK7 (Ser271 + Thr275)) Polyclonal Antibody, Unconjugated (orb6592) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Brain (mouse) Lysate at 40 ug, Primary: Anti-p-MEK7 (Ser271 + Thr275) (orb6592) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47kD, Observed band size: 48kD.

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Liver tissue lysates, Primary: Anti-Phospho-MEK7 (Ser271 + Thr275) (orb6592) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa.