Phospho-MEK6 (Ser202) Rabbit Polyclonal Antibody

SKU: orb6590

Description

Phospho-MEK6 (Ser202) Rabbit Polyclonal Antibody

Research Area

MAPK/ERK Signalling Pathway

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MEK6 around the phosphorylation site of Ser202 GI(p-S)GY
• Target: MAP2K6
• Molecular Weight: 37 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MAP kinase kinase 6; MAPK/ERK kinase 6; MAPKK6; MEK6; Mitogen Activated Protein Kinase Kinase 6; MKK 6; MKK6; PRKMK6; SAPKK3; Dual specificity mitogen activated protein kinase kinase 6; MKK 6; Dual specificity mitogen activated protein kinase kinase 6; EC 2.7.12.2; MP2K6_HUMAN.

Sample: Lane 1: Mouse Large intestine tissue lysates, Lane 2: Mouse Heart tissue lysates, Lane 3: Mouse Muscle tissue lysates, Primary: Anti-Phospho-MEK6 (Ser202) (orb6590) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kDa, Observed band size: 42 kDa.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-MEK6 (Ser202) (orb6590) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 38 kD.

Tissue/Cell: Rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MAPKK6 Polyclonal Antibody, Unconjugated (orb6590) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MAP2K6

UniProt

P52564