Phospho-MEK4 (Ser257 + Thr261) Rabbit Polyclonal Antibody

• Catalog Number: orb6585
• Category: Antibodies
• Description: Phospho-MEK4 (Ser257 + Thr261) Rabbit Polyclonal Antibody
• Target: MAP2K4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of Ser257/Thr261 VD(p-S)IAK(p-T)RD
• UniProt ID: P45985
• RRID: AB_10928921
• MW: 44 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MEK4(phospho S257+T261); SEK1/MKK4(phospho-Ser257+
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Muscle (Mouse) Lysate at 30 ug, Primary: Anti-p-MEK4 (Ser257+Thr261) (orb6585) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-MEK4 Polyclonal Antibody, Unconjugated (orb6585) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.