Phospho-MEK4 (Ser257) Rabbit Polyclonal Antibody

SKU: orb6583

Description

Phospho-MEK4 (Ser257) Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of Ser257 VD(p-S)IA
• Target: MAP2K4
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MAP2K4 | MEK4 (p-S257); p-MEK4; phospho-MEK4; JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1; MAPKK 4; MP2K4_HUMAN; MAP2K4; MAP kinase kinase 4; JNK-activating kinase 1; MAPK/ERK kinase 4 (MEK 4); SAPK/ERK kinase 1 (SEK1); Stress-activated protein kinase kinase 1 (SAPK kinase 1 | SAPKK-1 | SAPKK1); c-Jun N-terminal kinase kinase 1 (JNKK); 2.7.12.2; MP2K4_MOUSE; C-JUN N-terminal kinase kinase 1 (JNK kinase 1 | JNKK 1);

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MEK4 (Ser257)) Polyclonal Antibody, Unconjugated (orb6583) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

MAP2K4

UniProt

P45985