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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody

SKU: orb185135

Description

Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

Tested ApplicationsWB
Dilution rangeWB=1:500-2000
ReactivityHuman
Predicted ReactivityCanine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human MCM2 around the phosphorylation site of Ser53 DE(p-S)EG
TargetMCM2
Molecular Weight101 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

MCM4(phospho S53); MCM2(phospho-Ser53); MCM2(phospho Ser53); MCM2(phospho S54); p-MCM2(Ser53); p-MCM2(S53); rat MCM4(phospho S54); BM28; CCNL 1; CCNL1; CDC like 1; CDC like-1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; cyclin like-1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin(S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MCM2_MOUSE; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2(mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050.

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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: Jurkat (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: U-2OS (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-MCM2 (Ser53) (orb185135) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
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Phospho-MCM2 (Ser53) Rabbit Polyclonal Antibody (orb185135)

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