Phospho-MCAK (Ser95) Rabbit Polyclonal Antibody

• Catalog Number: orb185508
• Category: Antibodies
• Description: Phospho-MCAK (Ser95) Rabbit Polyclonal Antibody
• Target: Kif2c
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Equine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MCAK around the phosphorylation site of Ser95 RR(p-S)VN
• UniProt ID: Q99661
• MW: 81 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MCAK(phospho S95); p-MCAK(phospho S95); 4930402F02
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MCAK (Ser95)) Polyclonal Antibody, Unconjugated (orb185508) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.