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Phospho-MCAK (Ser95) Rabbit Polyclonal Antibody

SKU: orb185508

Description

Phospho-MCAK (Ser95) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman
Predicted ReactivityEquine, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthesised phosphopeptide derived from human MCAK around the phosphorylation site of Ser95 RR(p-S)VN
TargetKif2c
Molecular Weight81 kDa
PurificationAffinity purified by Protein A

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

MCAK(phospho S95); p-MCAK(phospho S95); 4930402F02Rik; ESTM5; KIF 2C; kif2c; KIF2C_HUMAN; Kinesein Family Member 2C; Kinesin family member 2C; kinesin like 6(mitotic centromere associated kinesin); Kinesin like 6; Kinesin like protein 6; Kinesin like protein KIF2C; Kinesin-like protein 6; Kinesin-like protein KIF2C; KNS L6; KNSL 6; KNSL6; MCAK; MGC11883; Mitotic centromere associated kinesin; Mitotic centromere-associated kinesin; OTTHUMP00000010066; X83316

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Phospho-MCAK (Ser95) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MCAK (Ser95)) Polyclonal Antibody, Unconjugated (orb185508) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-MCAK (Ser95) Rabbit Polyclonal Antibody (orb185508)

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
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