Phospho-MARCKS (Ser152 + Ser156) Rabbit Polyclonal Antibody

• Catalog Number: orb5566
• Category: Antibodies
• Description: Phospho-MARCKS (Ser152 + Ser156) Rabbit Polyclonal Antibody
• Target: MARCKS
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised phosphopeptide derived from rat MARCKS around the phosphorylation site of Ser152/156 or Ser159/163(human) RF(p-S)FKK(p-S)FK
• UniProt ID: P29966
• RRID: AB_10936936
• MW: 30 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MARCKS(phospho S152+S156); p-MARCKS(phospho S152+S
• Research Area: Calmodulin Pathway, Cardiovascular Research, Cytos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MARCKS (Ser152 + Ser156)) Polyclonal Antibody, Unconjugated (orb5566) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat Cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MARCKS (Ser152 + Ser156)) Polyclonal Antibody, Unconjugated (orb5566) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Spleen (Mouse) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-MARCKS (Ser152 + Ser156) (orb5566) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.