Phospho-MAPKAPK2 (Thr334) Rabbit Polyclonal Antibody

SKU: orb560142

Description

Phospho-MAPKAPK2 (Thr334) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Thr334 PQ(p-T)PL
• Target: MAPKAPK2
• Molecular Weight: 46 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MAPKAPK2(phospho Thr334); MAPKAPK2(phospho T334); MAPKAP Kinase 2(phospho T334); p-MAPKAP Kinase 2(phospho T334); MAPKAPK-2(phospho-Thr334); MAPKAPK2(phospho T334); MAP Kinase Activated Protein Kinase 2; MAPK activated protein kinase 2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK2; MAPK2_HUMAN; Mitogen Activated Protein Kinase Activated Protein Kinase 2; MK 2; MK2; MAPKAPK-2.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-MAPKAPK2 (Thr334)) Polyclonal Antibody, Unconjugated (orb560142) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-MAPKAPK2 (Thr334) Polyclonal Antibody, Unconjugated (orb560142) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

MAPKAPK2

UniProt

P49137