Phospho-MAK (Tyr159) Rabbit Polyclonal Antibody

• Catalog Number: orb185427
• Category: Antibodies
• Description: Phospho-MAK (Tyr159) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MAK around the phosphorylation site of Tyr159 TD(p-Y)VS
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 71 kDa
• Target: Mak
• UniProt ID: P20794
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MAK(phospho Tyr159); MAK(phospho Y159); dJ417M14.2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: k562 (human) lysate at 40 ug, Primary: rabbit Anti-phospho-MAK (Tyr159) (orb185427) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 71 kD, Observed band size: 71 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-MAK (Tyr159) (orb185427) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 71 kD, Observed band size: 71/66 kD.

Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-MAK (Tyr159) (orb185427) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 71 kD, Observed band size: 73 kD.

Tissue/Cell: human prostate tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated (orb185427) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated (orb185427) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.