Phospho-KSR1 (Ser404) Rabbit Polyclonal Antibody

SKU: orb2563511

Description

Phospho-KSR1 (Ser404) Rabbit Polyclonal Antibody

Research Area

Cell Biology, Kinases, MAPK/ERK Signalling Pathway, Tyrosine Kinases

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human KSR1 around the phosphorylation site of Ser404 TE(p-S)VP
• Target: KSR1
• Molecular Weight: 102 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KSR1(phospho S404); p-KSR1(phospho S404); BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1; KSR1_HUMAN; Positive Ras signaling mediator family member(86.4 kD); Positive Ras signaling mediator family member; RSU 2; RSU2; SR 31; SR31; Suppressor of activated let 60 Ras SUR3; Suppressor of Ras1 31.

Hela (H) cells were treated with or without Calyculin A (50nM 30) for 30 min, 25 µg total protein per lane of cell lysates probed with phospho-KSR1 (Ser404) polyclonal antibody, unconjugated (orb2563511) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Quick Database Links

Gene Symbol

KSR1

UniProt

Q8IVT5