Phospho-KMT6 (Ser21) Rabbit Polyclonal Antibody

SKU: orb157761

Description

Phospho-KMT6 (Ser21) Rabbit Polyclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human KMT6/EZH2 around the phosphorylation site of Ser21 VK(p-S)EY
• Target: EZH2
• Molecular Weight: 82 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EZH2 (phospho-S21); p-EZH2; phospho-EZH2; EZH2 (phospho-Ser21); ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2; Enx1h; mKIAA4065; EZH2_HUMAN; EZH2; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6; 2.1.1.356; EZH2_MOUSE;

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-EZH) Polyclonal Antibody, Unconjugated (orb157761) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EZH2

UniProt

Q15910