Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody

SKU: orb156299

Description

Phospho-ITGB2 (Thr758) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human CD18 around the phosphorylation site of Thr758 SA(p-T)TT
• Target: ITGB2
• Molecular Weight: 82 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Integrin beta-2; integrin subunit beta 2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta; Complement receptor C3 subunit beta;

Blank control: Raji cells (blue). Primary Antibody: Rabbit Anti-Phospho-CD18 (Thr758) antibody (orb156299), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CD18 (Thr758) Polyclonal Antibody, Unconjugated (orb156299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ITGB2

UniProt

P05107