Phospho-ITGB2 (Ser756) Rabbit Polyclonal Antibody

SKU: orb156298

Description

Phospho-ITGB2 (Ser756) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-P
• Dilution range: IHC-P=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human CD18 around the phosphorylation site of Ser756 FK(p-S)AT
• Target: ITGB2
• Molecular Weight: 82 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Integrin beta-2; integrin subunit beta 2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta; Complement receptor C3 subunit beta;

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-CD18 (Ser756) antibody (orb156298), Dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-CD18 (Ser756)) Polyclonal Antibody, Unconjugated (orb156298) at 1:200 overnight at 4°C, followed by a conjugated secondary (orb868589) at [1:200] for 90 minutes and DAPI staining of the nuclei.

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CD18 Polyclonal Antibody, Unconjugated (orb156298) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ITGB2

UniProt

P05107