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Phospho-IRS-2 (Ser731) Rabbit Polyclonal Antibody

Catalog Number: orb783332

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb783332
CategoryAntibodies
DescriptionPhospho-IRS-2 (Ser731) Rabbit Polyclonal Antibody
TargetIRS2
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityCanine, Equine, Mouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human IRS-2 around the phosphorylation site of Ser731 AS(p-S)PA
UniProt IDQ9Y4H2
MW147 kDa
Tested applicationsFC, ICC
Dilution rangeICC/IF=1:100, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesIRS-2(phospho-Ser731); IRS-2(phospho S731); Insuli
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NoteFor research use only
Phospho-IRS-2 (Ser731) Rabbit Polyclonal Antibody

Blank control (black line): SH-SY5Y. Primary Antibody (green line): Rabbit Anti-phospho-IRS-2 (Ser731) antibody (orb783332), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-IRS-2 (Ser731) Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-IRS-2 (Ser731)) polyclonal Antibody, Unconjugated (orb783332) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Phospho-IRS-2 (Ser731) Rabbit Polyclonal Antibody

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-IRS-2 (Ser731)) polyclonal Antibody, Unconjugated (orb783332) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.